The fertility of men is generally determined through the indicators of semen examination, so the details of semen examination are particularly important, of which there are the following four points: First, the time of collecting semen specimens, it is generally recommended that the patient abstain from sperm for about 3-5 days, if the time is too short or too long, such as more than half a month, the quality of sperm at this time will have obvious changes and inaccurate. Second, the number of times the semen specimen is collected. Since semen analysis is affected by a variety of factors, such as time, environment, and personal diet, a definitive diagnosis cannot be made based on the results of one semen, and it is generally recommended to review the test after an interval of 1-2 weeks. Thirdly, the method of semen collection is for the patient to come to the laboratory and be collected by himself with masturbation. It is not recommended to collect semen in the way at home or in other containers, which will affect the quality of sperm. Fourth, since the body temperature of the human body is 36.5°C, there will be a certain temperature difference after the sperm leaves the body, so if the outside temperature is too low during this period, it is best to use insulation measures, such as putting the semen specimen in the pocket of the underwear or quickly sending the semen specimen back to the laboratory, so that the quality of the sperm is not affected.