Ms. Xie was diagnosed with a right frontal lobe glioma 2 years ago and underwent surgery and gamma knife treatment in an outside hospital. 3 months ago, a review of the tumor revealed recurrence. Ms. Xie underwent a second surgery in an outside hospital. However, the result of this surgery was unsatisfactory, and there were still obvious residuals after the surgery. In order to seek further treatment, she was admitted to our hospital. After the multidisciplinary consultation, we concluded that the patient could be treated with radioactive particle implantation to eliminate the residual tumor. After careful preoperative preparation, we performed CT-guided radioactive particle implantation for glioma. Firstly, the lesion was localized under CT to determine the needle point, direction and depth. Then the patient’s dura mater was drilled through with a bone drill, and the 18G implantation needle was inserted into the lesion using a step-by-step approach under CT guidance. After checking the position of the needle tip, the radioactive particles were implanted. The operation went smoothly without any special discomfort and the patient’s vital signs were stable. No obvious cerebral hemorrhage or cerebrospinal fluid leakage was found in the postoperative CT examination. The patient returned to the ward safely. One week after the operation, MR examination showed that the tumor had shrunk. CT-guided radioactive particle implantation for glioma is a difficult treatment technique. Only a few large hospitals in China can carry out it. The successful implementation of this technique in our hospital has created a new hope for patients with recurrent or residual brain tumors after surgery, radiotherapy and gamma knife treatment.