With the current development of medicine, the use of specialized equipment, ultrasound guidance, etc., the puncture biopsy of lymph nodes is virtually free of medical hazards to the human body. Lymph node aspiration biopsy is an invasive procedure performed to clarify the pathological nature of lymph nodes when the cause of lymph node hyperplasia or enlargement is unknown, especially in lymph nodes with high suspicion of tuberculosis, malignant tumors, or lymphomas. Lymph node puncture biopsy has been widely used in clinical practice and is a commonly used examination tool. Generally, patients only experience slight pain or discomfort, which is within the normal tolerance range of the body and will not cause any harm to the body. Lymph node puncture biopsy patients, need to avoid water and spicy food, light diet, try to bed rest, do not do heavy labor.