Eating breakfast afterward has an effect on testing liver function. Checking liver function after breakfast may affect the accuracy of the corresponding test results. When the body ingests food, the liver, as the largest digestive organ in the whole body, secretes various enzymes to digest the food. Therefore, checking liver function after breakfast may easily lead to elevation of transaminases, while other indicators, such as protein, blood glucose, blood lipids, etc., may also be abnormal, resulting in false-positive results and misdiagnosis of diseases. In order to improve the accuracy of liver function results, it is generally recommended that blood be drawn after eight hours of fasting for liver function tests. It is also important to rest and avoid staying up late before the liver function test, as staying up late at night can also lead to elevated aminotransferases, as well as avoiding a high-fat diet before the blood draw. If there is any abnormality in the liver function test, it is recommended to consult a doctor in time to avoid delaying the condition.