Lymph node puncture surgery can be completed in about 10 to 20 minutes for a quick procedure and may take about 60 minutes for a slower one. Lymph node puncture surgery is also known as a traumatic procedure to remove lymph node tissue for pathological examination. How long it takes depends on the location of the operation and the size of the lymph nodes, etc. and cannot be generalized. If the lymph nodes are superficial and small in size, the operation is relatively simple and takes about 10 to 20 minutes to complete. If the lymph node is in a deeper location and also the size of the lymph node is relatively large, the operation will be more complicated and will take a longer period of time, probably around 30 to 60 minutes. The sample removed by puncture should be sent to the pathology department for examination as soon as possible. Lymph node puncture surgery should be carried out in a regular hospital to ensure safety, and the corresponding treatment plan should be adopted according to the nature of the pathology after the surgery.