The causes of calf muscle twitching are muscle overwork, electrolyte imbalance, cold, etc., as follows: First, muscle overwork: here muscle overwork is the most common cause of this type of symptoms, such as patients usually do not exercise, and suddenly carry out activities such as long-distance running, due to the overuse of the calf triceps, often leading to muscle spasm. Secondly, electrolyte imbalance: some patients will have such symptoms secondary to electrolyte imbalance, for example, after the patient has a lot of diarrhea, due to the loss of a large amount of inorganic salts, it may make the electrolyte imbalance in the body. Thereafter, the patient is prone to muscle excitability at the muscles of the calf, and the situation of convulsions. Third, cold: some patients may be cold at the calf, and then cause muscle twitching here.