HPV-53 positivity represents the presence of HPV-53 viral infection. HPV, also known as human papillomavirus, is categorized into low-risk and high-risk types, and HPV-53 is a high-risk type of HPV virus, which needs to be taken seriously. For women who are found to be HPV-53 positive for the first time, if the cervical TCT examination is fine and when there are no symptoms such as contact bleeding, women who are in good physical condition may be able to reduce or eliminate the viral infection by adjusting their daily habits such as improving immunity. Medication such as interferon can also be chosen under the guidance of a doctor to accelerate the conversion. If the positive infection persists or there are symptoms such as contact bleeding, and clinical speculum examination reveals obvious lesions on the cervix, it is recommended that a cervical biopsy be performed to rule out the presence of cervical cancer or pre-cancerous lesions, and according to the results of the pathology, appropriate treatments such as surgical treatments, radiotherapy, chemotherapy, and so on, should be chosen. The common way of HPV infection is sexual transmission. In daily life, we should pay attention to cleanliness, avoid early sexual life or multiple sexual partners, and exercise appropriately to enhance physical fitness and prevent viral infection.