Drinking water can have an effect on cholesterol measurement. Total cholesterol is the sum of cholesterol contained in all lipoproteins in the blood. The liver is the main organ for synthesizing and storing cholesterol, and specimens sent for cholesterol testing should be fasted for 12 to 14 hours before blood collection, not drinking alcohol within 24 hours, and avoiding the effects of taking relevant medications. Drinking water can still cause gastrointestinal peristalsis, so that the liver is involved in digestion, thus affecting the accuracy of cholesterol testing. Other factors affecting the cholesterol test include high bilirubin, which can cause significant negative interference with the results; hemolysis, which can cause positive interference; high blood uric acid, which can also cause negative interference; and a large number of reducing drugs, such as vitamin C, phenolsulfonyl ethylamine, ipecac hydrochloride, and compound Salvia divinorum, which can interfere with the reaction and make the results low. It is recommended to consult the doctor before the relevant examination, do not make changes on your own, so as not to affect the accuracy of the results. Other factors affecting cholesterol measurement include high bilirubin, which can cause significant negative interference with the results (Wang Zhong et al., “Bilirubin Interference with Cholesterol Detection”); hemolysis, which can cause positive interference, but the interference can be ignored when the Hb is below 1g/L (mentioned in “The Impact of Hemolysis of Specimens on the Results of Biochemistry Tests and the Related Countermeasures”); high blood uric acid, which can cause negative interference; and high amounts of reductive drugs, such as Vitamin C, phenolsulfonyl ethylamine, ipecac hydrochloride, compound salvia, etc., can also interfere with the reaction and make the results low (reducing drugs have strong reducing properties, and negatively interfere with the results of enzyme-coupled Trinder reaction for cholesterol measurement).