Blood sampling before puncture needs to clarify the presence of infectious diseases, inflammatory diseases and coagulation system diseases. 1. First of all, it is necessary to clarify whether there are infectious diseases such as hepatitis B, hepatitis C, syphilis or HIV, etc. If there are, such patients should be separated from routine surgery patients for the puncture procedure, and the required surgical instruments need to be completely sterilized; 2. Secondly, the patient’s blood routine, liver and kidney function should be perfected to clarify whether there are other special diseases and inflammatory diseases, if there are inflammatory diseases, puncture should not be performed; 3. Finally, improve the patient’s coagulation function, and make clear whether there are bleeding disorders, if there are bleeding disorders or other special types of blood diseases, the puncture surgery should not be carried out, denying that it is easy to trigger the inability to stop bleeding or hemorrhage and other diseases after the puncture. Therefore, it is necessary to improve the blood tests before the puncture surgery, and the tests required for different types of puncture surgeries are different, which should be completed under the guidance of physicians.