About 5 ml of mid-stream urine from a continuous urine stream was collected in a test tube and sent to the laboratory for examination. Bacteria of epididymitis are mostly infected by retrograde transmission from the vas deferens to the epididymis, and the common causative organism is Escherichia coli. When doing mid-stream urine drug sensitivity, it should be noted that when collecting urine, it should be ensured that the urine flow is continuous and cannot be collected after interrupting urination. The specimen should not be left for too long after collection of urine to avoid contamination and inaccurate results. Epididymitis is often caused by urinary tract infections and prostatitis, sexually transmitted diseases and vesiculitis spreading retrogradely through the ureter, and is most common in young and middle-aged people. Treatment needs to focus on the treatment of the primary disease. For epididymitis, 0.5% lidocaine can be used to seal the spermatic cord to minimize pain, broad-spectrum antibiotics can be used, and incision and drainage are needed when abscesses form.