1. Evaluation of hearing loss level: Accurate evaluation of pure tone hearing threshold before treatment is very important. Because the subjective hearing test results are affected by many factors such as natural fluctuation of hearing, patient’s emotion, testing equipment, whether the non-testing ear is correctly covered and the operating mode of the examiner, one or two tests are often unable to determine the true level of hearing loss, which can cause misjudgment or inability to scientifically evaluate the treatment effect, thus failing to adjust the treatment plan in time to achieve the expected effect. 2. To confirm the diagnosis of iron deficiency deafness, routine blood, biochemical and immunological tests are generally required to clarify the occurrence of iron metabolism disorders. For example, the measurement of daily fluctuation difference of serum iron requires the patient to fast for more than 4 hours before the examination, to detect the difference between the highest and lowest values of the physiological cycle, and to assess the degree of association between deafness and iron metabolism disorder according to whether the fluctuation difference is normal or whether it is inverted, and whether iron metabolism disorder has affected related trace elements as well as immunomodulatory factors. 3. After the diagnosis of iron deficiency deafness is confirmed, an individualized treatment plan needs to be drawn up as soon as possible. Due to the large differences in gender, age, physical condition, etiology, duration of the disease, degree of hearing loss, lifestyle and diet, it usually takes several days to several weeks to observe the patient’s response and degree of adaptation to the medication, as well as changes in hearing, to adjust the medication and dosage in a timely manner, and finally to formulate the first stage treatment plan.