Tubal fluid reflux can be determined by hysterosalpingography, magnetic resonance imaging (MRI) examination and hysterolaparoscopy. 1. Hysterosalpingography: X-ray or ultrasound examination is performed by injecting contrast medium into the uterine cavity through the cervix to determine whether the fallopian tubes are open or not according to the visualization of the uterine cavity and the fallopian tubes. 2. Magnetic resonance imaging (MRI) examination: MRI hysterosalpingography can not only show the smoothness of the uterine cavity and fallopian tubes, but also show the endometrium and myometrium signals very well. 3. Hystero-Laparoscopy: Hystero-Laparoscopy is the gold standard for the diagnosis of tubal patency, but it is an invasive surgery, and most of the patients need general anesthesia. Hystero-Laparoscopy can investigate the umbilical end of the fallopian tubes and the surrounding conditions. If the results of the above tests are abnormal, it is recommended to consult a doctor for a clear diagnosis and targeted treatment or therapy.