The third vaccination generally refers to the third booster shot of the New Crown vaccine, and nucleic acid can be done 48 hours after the vaccination. The third vaccination refers to the booster shot of the new crown vaccine, which belongs to the supplemental vaccine to strengthen the viral antibody titer after the new crown vaccination has been regularly completed, and the vaccination can make the new crown virus antibody immunity greatly improved. After vaccination, the residual viral nucleic acid fragments inside the vaccine may cause false-positive results for the patient’s next nucleic acid test. It is best to perform the nucleic acid test at an interval of more than 48 hours to ensure that the correct test results are obtained after the residual nucleic acid fragments are removed from the vaccinated person. Therefore, before undergoing nucleic acid testing, it is best to consult a medical professional on related issues to prevent the occurrence of adverse reactions during the vaccination process.