Bile in bile enzyme separation refers to bilirubin, and enzyme refers to transaminase. Bile enzyme separation refers to a persistent and significant increase in bilirubin index while transaminase index is decreasing. Bile enzyme separation occurs in patients with severe hepatitis and liver failure in the state of massive and submassive liver necrosis. After the hepatocytes are extensively destroyed and the transaminases in the hepatocytes are released into the blood in large quantities, there are no more subsequent hepatocytes available for releasing transaminases, which will gradually decrease as the transaminases are catabolized and metabolized, while the bilirubin enters the blood circulation in large quantities after the liver structure is extensively destroyed, resulting in increasingly high bilirubin indexes. Separation of bilirubin enzymes is a manifestation when the liver is in a severe state, often leading to clinical death due to liver failure.