The five indicators of bone metabolism include osteocalcin, type I precollagen amino-terminal prepeptide, parathyroid hormone, 25-hydroxyvitamin D3, and type I collagen cross-linked C-terminal peptide. 1. Osteocalcin: It is a protein synthesized by osteoblasts. Its level reflects the activity of osteoblasts and active bone formation. A high level of osteocalcin is indicative of bone synthesis and is common in diseases such as hyperparathyroidism. A low level of osteocalcin indicates calcium deficiency in most patients. 2. Amino-terminal pre-peptide of type I pre-collagen: the level of this index can reflect the rate of type I collagen synthesis in bone tissue, as well as the activity level of osteoblasts and the level of bone formation. Elevated levels can be seen in children’s development, bone tumors and other conditions. 3. Parathyroid hormone: It is a hormone secreted by parathyroid glands, and its function is to increase blood calcium and blood phosphorus. Elevation of this index can be seen in hyperparathyroidism and renal osteodystrophy; decrease of this index can be seen in hypoparathyroidism. 4.25-Hydroxyvitamin D3: a form of vitamin in the body. A decrease in this level indicates a vitamin D deficiency, which can be seen in vitamin D-related osteochondrosis, some gastrointestinal disorders, and so on. A high level of this indicator is rare. 5. C-terminal peptide of type I collagen cross-linking: it reflects the activity of osteoclasts and the level of osteolytic activity, and its elevation can be seen in osteoporosis, bone metastatic cancer and so on. For patients who have undergone the five bone metabolism tests, the relevant reports should be submitted to the physician, who will make a comprehensive diagnosis and carry out relevant treatment according to the patient’s condition and the results of the reports. Patients should not make self-diagnosis based on the checklist.