Clinically there is no most accurate signal of successful frozen blastocyst implantation, generally after successful frozen blastocyst implantation, there will be menopause, elevated human chorionic gonadotropin, and gestational sacs can be seen in the uterine cavity and other manifestations. 1. Amenorrhea: amenorrhea is the first manifestation of pregnancy, successful pregnancy after the implantation of blastocysts, estrogen and progesterone levels in the pregnant woman’s body increase significantly, pregnant women will not menstruate. 2. Elevation of human chorionic gonadotropin: after successful implantation of frozen blastocysts, the chorionic gonadotropin secreted by the trophoblast cells of the embryo will rise rapidly, and the secretion of human chorionic gonadotropin in pregnant women will be obviously increased. 3. Gestational sac can be seen in the uterine cavity: when pelvic ultrasound examination is performed, the development of gestational sac can be seen in the uterine cavity, and it can be determined as intrauterine pregnancy. After frozen blastocyst transplantation, you should pay attention to light diet, balanced nutrition, pay attention to rest, keep your mood relaxed, and prohibit sexual life.