Trypanosoma cruzi is an African human trypanosomiasis, also known as sleeping sickness, which is a vector-borne parasitic disease. Trypanosoma brucei and Trypanosoma brucei Rhodesia are the causative agents of African trypanosomiasis or African trypanosomiasis, and the vector insects are tsetse flies. Trypanosoma gambiae is found along rivers or along forests in West and Central Africa, while Trypanosoma rhodesiae is found in shrub and vegetation zones in the savannas and lakeshores of East Africa. Africantrypanosomiasis (Africansleepingsickness) has three clinical stages, the first of which is the invasion of the skin, causing varying degrees of hardness and nodules, which evolve into trypanosomatous chancre, which occurs in about one-third of patients and is often found on the exposed skin where the tsetsefly bites. The chancre occurs in about one-third of patients and is often found on the exposed skin where the tsetsefly bites and lasts for about 3 weeks. The second period is the hemolymph period, when symptoms such as periodic fever and parasitemia occur, including lethargy, swelling of lymph nodes in the back of the neck, joint pain, headache, and red rash on the trunk, and myocarditis, and jaundice due to hemolysis and liver damage. Once the brain is invaded, the disease will enter the third stage of meningoencephalitis, with headache, insomnia, movement disorders and behavioral disorders, and other symptoms including general weakness, dramatic loss of appetite and weight loss. The test items for trypanosomiasis are: 1. Cerebrospinal fluid Cerebrospinal fluid is a colorless and transparent liquid filled in the ventricles, the subarachnoid space and the central canal of the spinal cord. Cerebrospinal fluid is produced by the choroid plexus in the ventricles and is similar in nature to plasma and lymphatic fluid, being slightly viscous. Therefore, when the central nervous system is damaged, the detection of cerebrospinal fluid becomes one of the important auxiliary diagnostic tools. 2.Cerebrospinal fluid routine test Cerebrospinal fluid is a special body fluid that envelops and circulates around the brain tissue and spinal cord of the nervous system, and plays a vital role in the protection, nutrition and metabolism of the brain. 3.Parasitic blood test Parasitic blood test is an auxiliary test used to check for parasites. Parasites can be found in the blood and blood cells. These protozoa and filarial parasites are found in human blood and red blood cells and need to be confirmed by blood tests or bone marrow tests. The common methods used are blood smears (thin and thick blood smears) and bone marrow aspiration. It is an auxiliary test used to check for parasites. Routine blood test is the most general and basic blood test. Routine blood tests are usually performed with peripheral blood, such as blood from the fingertips and earlobes. After the blood cell analysis instrument, the computer reports the results, and this item has become a practice of examining patients. Blood consists of two major parts, liquid and tangible cells, and routine blood tests are performed on the cellular part of the blood. Blood has three different functions of cells – red blood cells (commonly known as red blood cells), white blood cells (commonly known as white blood cells), and platelets. Diseases are determined by observing changes in number and morphological distribution. It is one of the common auxiliary tests used by doctors to diagnose the disease. 5.Serum α2-macroglobulin (α2-MG) α2-MG is the largest molecular weight protein in plasma and is synthesized by hepatocytes and monocyte-macrophage system. It has a half-life of about 5 days and acts as an enzyme inhibitor. It regulates extracellular protein hydrolysis and also stimulates lymphocyte and granulocyte development. The measurement of α2-macroglobulin in blood has some significance for the diagnosis of liver and kidney diseases.