Trypanosoma cruzi is an African human trypanosomiasis, also known as sleeping sickness, which is a vector-borne parasitic disease. Trypanosoma brucei and Trypanosoma brucei Rhodesia are the causative agents of African trypanosomiasis or African trypanosomiasis, and the vector insects are tsetse flies. Trypanosoma gambiae is found along rivers or along forests in West and Central Africa, while Trypanosoma rhodesiae is found in shrub and vegetation zones in the savannas and lakeshores of East Africa. Africantrypanosomiasis (Africansleepingsickness) has three clinical stages, the first of which is the invasion of the skin, causing varying degrees of hardness and nodules, which evolve into trypanosomatous chancre, which occurs in about one-third of patients and is often found on the exposed skin where the tsetsefly bites. The chancre occurs in about one-third of patients and is often found on the exposed skin where the tsetsefly bites and lasts for about 3 weeks. The second period is the hemolymph period, when symptoms such as periodic fever and parasitemia occur, including lethargy, swelling of lymph nodes in the back of the neck, joint pain, headache, and red rash on the trunk, and myocarditis, and jaundice due to hemolysis and liver damage. Once the brain is invaded, the disease will enter the third stage of meningoencephalitis, with headache, insomnia, movement disorders and behavioral disorders, and other symptoms including general weakness, dramatic loss of appetite and weight loss. The test for trypanosomiasis: 1. Smear examination: Take blood smear for microscopic examination. The patient’s blood can be stained and examined microscopically. Lymphatic fluid, cerebrospinal fluid, bone marrow aspirate and lymph node aspirate can also be taken for smear examination. 2, serological diagnostic methods: commonly used enzyme-linked immunosorbent assay (ELISA), indirect fluorescent antibody test, and indirect hemagglutination test and other methods. 3.Molecular biology methods: PCR, DNA probe technology.