Embryos are matched on the same day as egg and sperm retrieval. IVF egg retrieval and sperm retrieval are usually performed on the same day. Fresh eggs and sperm help to safeguard the fertilization and embryo rates. If the male partner is unable to retrieve sperm on the day of egg retrieval due to various factors, the sperm will be retrieved in advance and then frozen for storage and thawed for use on the day of egg retrieval. The retrieved sperm and eggs will be placed in a petri dish for in vitro fertilization on the same day. The laboratory technician will observe the fertilization, division and embryo formation of the sperm and egg every day, and will usually obtain transferable embryos on the 3rd-4th day. The woman’s suitability for transfer is assessed at that time, and if transfer is not possible, the embryos may be frozen. If the embryos need to be cultured into blastocysts, the quality of the embryos needs to be assessed and continued to be cultured in vitro. It is recommended that both spouses go to the hospital for a pre-pregnancy eugenic health checkup to assess whether they are physically fit to get pregnant. Quit smoking and drinking, develop good living habits, maintain good health, avoid prolonged exposure to radiation or pollution, and get out of toxic and hazardous work environments. Women should take oral folic acid starting 3 months before the planned pregnancy to prevent neural tube defects.