When the value of transaminase exceeds 100 U/L, it is more than twice the normal value, which indicates the existence of obvious liver cell damage, most likely caused by organic diseases, such as viral Hepatitis, fatty liver, alcoholic liver, autoimmune liver disease, and even primary liver cancer. These diseases sometimes do not have obvious clinical symptoms in the early stages and are therefore easily missed and misdiagnosed. For aminotransferases caused by physiological factors, such as strenuous exercise or staying up all night, the aminotransferases are usually only mildly elevated and will quickly return to normal after the triggers are removed. Therefore, when the aminotransferase value reaches 100 U/L, it is necessary to further improve the relevant tests to clarify the existence of the disease, and the situation cannot be explained by simply staying up late.