People who usually can’t use stem cells include: shock, advanced malignant tumors, severe systemic infections, people with coagulation disorders, and so on. Usually contraindications to stem cell therapy include: 1. Shock or systemic organ failure; 2. patients with advanced malignant tumors, especially brain tumors; 3. systemic infection or local serious infection before recovery from anti-infection treatment (levofloxacin); 4. patients with combined heart, lung, liver, kidney and other important organ dysfunction; 5. coagulation dysfunction; 6. Positive serologic tests (e.g., AIDS, hepatitis B, syphilis, etc.); 7. non-neurological disorders or those who have not been clearly diagnosed. 8. In addition highly allergic or with a history of severe allergies; abnormal and do not cooperate with the examination; as well as some other patients who are not suitable for stem cell therapy should not use stem cells for treatment. Therefore, when conducting research and application of stem cell transplantation therapy, patients need to be screened and specific treatment should be carried out under the guidance of physicians.