The 6 sex hormones, as the name implies, are estradiol, follicle stimulating hormone, luteinizing hormone, prolactin, progesterone and testosterone. Estrogen is an 18-carbon hormone, including estradiol, estrone and estriol, the most powerful one is estradiol (E2), which is secreted by the ovaries, that is why the first item of the 6 sex hormones test is estradiol. The serum concentration of estradiol is less than 50 pg/ml in the early follicular phase, and can reach 200-300 pg/ml as the follicle develops, especially before ovulation. FSH and Luteinizing Hormone (LH) Both hormones are secreted by gonadotropin cells of the pituitary gland and are glycoprotein hormones; FSH plays a key role in follicle recruitment and selection of the dominant follicle and is necessary for follicle development; LH promotes final maturation of the oocyte and ovulation before ovulation. Prolactin (PRL) is secreted by the prolactin cells of the pituitary gland and its main role is to promote milk secretion after delivery. During the non-pregnancy period, PRL inhibits the secretion of FSH and LH in a certain way, so abnormal menstruation may occur in patients with high prolactin. 4.Progesterone (P) is a 21-carbon steroid hormone, which is the main hormone secreted during the luteal phase. Its main role is to let the endometrium transform into the secretory phase after the action of estrogen in order to facilitate embryo implantation, and if implantation is successful, a certain level of progesterone is needed to maintain pregnancy. 5. Testosterone (T) is a kind of androgen, in addition to androstenedione and dehydroepiandrosterone. Part of testosterone is secreted by the ovaries, and part of it is converted from androstenedione. Testosterone is transformed into estrogen through certain pathways under the action of FSH, so it is said that testosterone is the predecessor of estrogen. Sex hormone 6 is a necessary test to evaluate ovarian function and diagnose some endocrine diseases, and it is usually required to be checked 2-4 days during menstruation. For example, elevated E2, elevated FSH and decreased LH during menstruation indicate decreased ovarian function. Elevated PRL may lead to menstrual disorders or even amenorrhea, and elevated P in the early follicular phase may lead to the exclusion of pregnancy and the use of progesterone. In addition, many of the 6 sex hormone tests affect each other, so you cannot diagnose a disease by checking one of them alone or by elevating one of them alone. Therefore, patients with menstrual disorders, pregnancy preparation, etc. should have their sex hormone 6 tests checked and consult a reproductive endocrinologist when the results are available.