Non-gonococcal urethritis is still treated with sensitive antibiotics in terms of treatment. Non-gonococcal urethritis refers to pathogens other than Neisseria gonorrhoeae, of which Chlamydia trachomatis and mycoplasma infections are common, and most remain sexually transmitted infections. The symptoms of non-gonococcal urethritis are similar to gonorrhea urethritis in that they are often accompanied by painful urination and urethral discharge that is yellowish or purulent in color, and sometimes increased leucorrhea in women. 20%-50% of the population does not have obvious clinical symptoms and is often detected by physical examination. Treatment is still based on the use of appropriate and sensitive antibiotics for the pathogenic bacteria. Mycoplasma and Chlamydia cultures plus drug sensitivity tests are used to identify sensitive antibiotics for treatment. For example, macrolide antibiotics, such as azithromycin or roxithromycin, are preferred for mycoplasma and chlamydia trachomatis. In addition to the application of antibiotics, general treatment such as drinking plenty of water and keeping clean is also important. Antibiotics should be applied in adequate doses and courses during treatment. Of course, treatment and prevention should go hand in hand, and the patient’s towels, underwear or bath tubs should be separated, and sexual partners need to be treated at the same time. The criteria for cure are disappearance of clinical symptoms for more than one week, negative urine sediment microscopy, and negative urethral or cervical smear and culture. In summary, the treatment of non-gonococcal urethritis is still based on antibiotic treatment, and the pathogenic bacteria infection needs to be clarified before treatment, and timely symptomatic treatment.