Hepatitis B virus survival time in vitro is generally long, usually about 7 to 15 days in a regular in vitro environment. The survival time is different in different environments. The survival time of hepatitis B virus in vitro is mainly affected by factors such as temperature, humidity and acidity. Generally speaking, hepatitis B virus can survive for about 7-15 days at room temperature, about 6 months in serum, and even decades at -20 degrees Celsius. Hepatitis B virus does not tolerate high temperature, and can be inactivated by heating at 100 degrees for 10 minutes or 65 degrees for 10 hours. In addition, the higher the humidity of the environment, the longer the survival time of hepatitis B virus will be. Strong acids and bases can inactivate the hepatitis B virus very quickly. It should be noted that patients suspected of having hepatitis B virus infection should go to the hospital for examination, clear diagnosis and active treatment.