Yan Wenming, Department of Radiotherapy, Affiliated Hospital of Inner Mongolia Medical University
[Abstract] Objective To investigate the correlation between plasma ultrasensitive c-reactive protein and diabetes mellitus and diabetic patients with atherosclerosis Methods Immunoturbidimetric method was used to determine plasma hs-CRp levels in 30 patients with type 2 diabetes mellitus and 35 patients with type 2 diabetes mellitus combined with bilateral lower extremity atherosclerosis, and to compare with 20 normal healthy controls. The plasma hs-CRp levels in patients with type 2 diabetes were significantly higher than those in normal controls (p0.05). In the healthy control group, 20 healthy adults without heart, liver, or kidney diseases were selected from our outpatient medical checkup.
Methods
(1) The plasma hs-CRP levels of 30 patients with type 2 diabetes mellitus and 35 patients with diabetes mellitus combined with bilateral lower limb atherosclerosis were measured by immunoprojection turbidimetry and compared with 20 normal healthy controls.
(2) Blood glucose, serum, total cholesterol, triglyceride, LDL and HDL were measured by enzymatic assay in diabetic patients and healthy controls, respectively.
1.3 Statistical treatment
The results were expressed as mean + standard deviation (x+s), and t-test was used for comparison between groups.
2. Results
Serum hs-CRP levels were significantly higher in type 2 diabetic patients than in normal controls (p>0.05), and the group with lower limb atherosclerosis was significantly higher than the group without lower limb atherosclerosis (p>0.01). The plasma hyper-sensitive c-reactive protein levels were positively correlated with LDL, cholesterol and triglycerides and negatively correlated with HDL.
3. Discussion
Ultrasensitive c-reactive protein hs-CRP is a very sensitive acute temporal protein and a highly sensitive indicator of inflammation. Normally present in trace amounts in plasma, hs-CRP increases significantly in response to collective febrile illness, various inflammatory conditions and trauma. Many studies now support that atherosclerosis is a chronic inflammatory disease, and there is a direct link between plasma hs-CRP levels and the onset, progression and prognosis of atherosclerosis. The complications of coronary heart disease, cerebrovascular disease, renal atherosclerosis, and limb atherosclerosis due to atherosclerosis are significantly higher in diabetic patients than in non-diabetic patients, and peripheral limb atherosclerosis is often dominated by arterial lesions in the lower limbs. In this study, we measured the plasma hs-CRP levels in diabetic patients, diabetic patients with combined lower limb atherosclerosis and healthy controls, and found that the plasma hs-CRP levels in 65 diabetic patients were higher than those in normal controls, and the plasma hs-CRP levels in the diabetic patients with combined lower limb atherosclerosis group were higher than those in the group without combined lower limb atherosclerosis. This further confirmed that hs-CRP may be an influential factor in the development of diabetes mellitus and atherosclerosis.
In the previous studies on the correlation between hs-CRP and diabetes mellitus and atherosclerosis, most of the studies on the correlation between hs-CRP and carotid artery intima, the present study investigated the relationship between hs-CRP and lower extremity atherosclerosis to investigate the effect of hs-CRP on atherosclerosis in diabetic patients from another perspective.
In addition, diabetic patients have different degrees of lipid metabolism disorders (hypertriglyceridemia, hypercholesterolemia, and low HDL), and the increase of plasma cholesterol and triglyceride can indirectly promote the development of plaque through fibrin. Therefore, we believe that hs-CRP and lipid metabolism disorders together contribute to the process of atherosclerosis.
Even mildly elevated plasma hs-CRP concentrations within normal limits are associated with an increased risk of cardiovascular disease, and c-reactive protein may become a useful and increasingly interesting indicator for routine cardiovascular risk assessment, and the relationship between c-reactive protein and atherosclerosis needs to be investigated in more depth.
[Ref.]
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3. Qian Lirong, Treatment of dyslipidemia in diabetic patients [J], New Advances in Clinical Lipidology, 2002.2(2):1