The five liver function tests are obtained by blood sampling, which is a method of checking various indicators related to liver function and metabolism through various chemical reagents, mainly including glutathione, glutamic oxalacetic transaminase, total bilirubin, direct bilirubin and indirect bilirubin. Among them, glutathione and glutathione are very sensitive indicators of acute hepatocyte necrosis and injury. Total bilirubin, direct bilirubin, and indirect bilirubin can help to identify and diagnose the type of jaundice. The five liver function tests are easily disturbed by some factors, so the following points need to be noted before the test: i. Do not eat or drink before the test, and you need to keep an empty stomach for 8-12 hours. Second, the night before the examination do not drink, do not eat spicy and stimulating food, do not eat greasy food, to be light. Third, the night before the examination do not stay up late, do not take drugs, otherwise the possible increase in transaminases, affecting the results of liver function tests.