The four liver function tests are usually obtained by blood sampling, and it mainly includes glutathione, total bilirubin, direct bilirubin and indirect bilirubin. An increase in ghrelin can be seen in various acute and chronic hepatitis, drug-related liver injury, and alcohol-related liver injury. Some diseases of the hepatobiliary system, such as gallbladder stones and common bile duct stones, can also lead to elevated transaminases. In addition, non-pathological factors, such as staying up late, drinking alcohol, and strenuous exercise, can also lead to a mild increase in transaminases. Total bilirubin, direct bilirubin and indirect bilirubin, the three main indicators reflecting jaundice, are mainly obstructive jaundice in terms of hepatobiliary surgery, which may have elevated total bilirubin, direct bilirubin and indirect bilirubin, with elevated direct bilirubin predominating.