Platelet mono collection is the process of separating platelets from a donor’s body through a blood cell separator and storing them in a platelet bag to supply the patient’s needs. It takes 12 cycles of platelet mono collection to isolate a therapeutic amount of platelets. With single platelet collection, it is possible to provide a constant supply of platelets needed by patients in the clinic. By transfusing a therapeutic amount of platelets, the platelet count of healthy adults can be increased by about 30,000. In the case of pediatric patients, the platelet count can be increased by about 60,000. Therefore, platelet mono is the most common type of component blood donation and transfusion, and it can save blood supply and improve the utilization rate of blood by platelet mono, which is a more common way to donate blood in clinical practice and is safer. Because platelet collection devices are now disposable and are performed in closed tubes, there is no significant harm to the human body.