If two transfers do not result in implantation, you need to further investigate your physical condition, including endometrial tolerance, reproductive immunity and chromosomes. 1. Endometrial tolerance: endometrial tolerance can be determined by removing the endometrium for pathology, and low tolerance will affect embryo implantation. 2. Reproductive immunity test: the condition of reproductive immunity system can be checked by blood sampling, which mainly includes anti-sperm antibody, anti-ovarian antibody, anti-endometrial antibody and anti-cardiolipin antibody, and the abnormality of the corresponding indexes will also affect the embryo’s implantation. 3. Chromosome: some people have abnormal chromosome status, even if the embryo is formed in the early stage, it can not develop normally, resulting in the embryo not implanting. If the embryo does not implant twice, it is necessary to consult a doctor in time for observation, and after diagnosing the condition through clinical examination, carry out standardized treatment under the guidance of the clinician, so as to prepare for the next implantation.