Novel Coronavirus Pneumonia is a respiratory transmitted disease that typically presents with fever and cough, and differs from other pneumonias in that it is specifically demonstrated on a chest CT. Experienced doctors are able to determine the likelihood of the disease by CT, but the definitive diagnosis is made by viral nucleic acid testing. Virus isolation can also be done in research institutions where it is available, and in most medical institutions the diagnosis is made by nucleic acid testing using PCR, which, if positive, confirms the diagnosis of infection with novel coronavirus pneumonia. Most of this test is done by throat swab. If it is negative, but the symptoms are very supportive of novel coronavirus pneumonia, it is recommended to take deep respiratory secretions, such as sputum and alveolar lavage, which will have a higher positive rate and is the most accurate and reliable test. However, due to sampling limitations, for example, in early and late stages of infection, when the viral load in the pharynx has decreased, false negatives are possible. In addition, some infected patients have been found to be positive for nucleic acids in urine and feces, which may further explore the significance of the positivity of specimens from the above sites and whether they can be used for diagnosis or exclusion. Serum antibody testing has recently been conducted in various healthcare facilities and may also be used as a diagnostic option. Source: Dr. Yurai