Since tuberous sclerosis can involve multiple systems or organs, ancillary tests are very important for the diagnosis of tuberous sclerosis and monitoring changes in the disease. 1.Head MRI or CT: Routine examination in the process of diagnosing TSC. To monitor the progression of giant cell astrocytoma, it is recommended that children be reviewed every 1 to 3 years (at least once a year if the giant cell astrocytoma is >1 cm or if the child’s expression is affected by intelligence problems). 2. EEG: For asymptomatic children younger than 2 years old, it is recommended to review the EEG every 6 weeks for early initiation of antiepileptic treatment. For patients with epilepsy, the frequency of EEG review depends on the condition of the epilepsy. 3. Evaluation of psychomotor development: When diagnosing TSC, an evaluation should be performed. Before going to school, a further evaluation may be performed. 4.Skin examination: When diagnosing TSC, careful examination should be performed. 5.Ophthalmologic examination: When diagnosing TSC, it should be carefully examined. 6.Cardiac ultrasound and electrocardiogram: When diagnosing TSC, especially for younger children. 7. Chest CT and pulmonary function: Chest CT should be performed even for young female patients without pulmonary symptoms. Women with lymphangioleiomyomatosis should have their pulmonary function rechecked every 6-12 months. 8. Renal ultrasound and renal function: All patients should undergo renal ultrasound at the time of diagnosis of TSC. Older children and adults should be rechecked every 1~3 years. When children have polycystic kidney and adult patients have severe renal damage, renal clearance should be performed.