I. Indications 1.Symptomatic gallbladder stones. 2.Gallbladder stones with comorbidities: even if there is no symptom, but with diabetes, cardiopulmonary dysfunction disease, should be operated in the stable period. 3. Gallbladder stones with complications: combined with acute and chronic cholecystitis, common bile duct stones, biliary pancreatitis and other complications, suitable for laparoscopic surgery. 4. Gallbladder stones with increased chance of gallbladder cancer: age > 60 years, huge stones (diameter > 2cm), ceramic gallbladder, etc. Absolute contraindications: 1. Those with severe cardiopulmonary insufficiency and unable to tolerate anesthesia, pneumoperitoneum and surgery. 2.With coagulation dysfunction. 3.Acute cholecystitis with serious complications, such as: gallbladder gangrene, perforation. 4.With acute severe cholangitis or acute gallstone pancreatitis. 5, Gallbladder cancer or suspected gallbladder cancer. 6.Chronic atrophic cholecystitis, gallbladder volume <4.5cm×1.5cm, wall thickness >0.5cm (measured by ultrasound). 7.Severe cirrhosis with portal hypertension. 8.Patients with middle or late pregnancy. 9, with abdominal infection, peritonitis. 10, with diaphragmatic hernia. Relative contraindications: 1. Acute attack of calculous cholecystitis. 2.Chronic atrophic calculous cholecystitis. 3, common bile duct stones and obstructive jaundice. 4.Mirizzi syndrome, gallbladder neck stone impaction. 5, History of previous upper abdominal surgery. 6, Morbid obesity. 7, Extra-abdominal hernia.