At present, various interventional minimally invasive techniques play an important role in the treatment of tumors, including temperature-based physical ablation techniques (radiofrequency ablation, microwave ablation, cryoablation, etc.), chemical ablation, radioactive particle implantation, etc. However, temperature-based physical ablation is non-selective to the destruction of tissue structures, and will destroy important structures such as blood vessels, bile ducts and nerves in the ablation area, which limits the treatment of tumors located in these important structures, especially those located in the pancreatic and hilar regions. The indications for radioactive particle implantation are broader than those for temperature-based physical ablation, but in some cases, the ideal particle implantation is difficult to be achieved. As a newest tumor ablation technology, NanoKnife can treat tumors located in special areas and broaden the indications for tumor treatment, which shows great application prospects. Nano knife ablation technology is a new non-temperature physical ablation technology developed based on the principle of irreversible electroporation. Its basic principle is that the electrode piercing to the lesion generates high-voltage direct current pulses, which can make nano-scale irreversible pores in the cell membrane and make the tumor cells apoptosis so as to achieve the purpose of tumor cell ablation. This technique does not rely on traditional temperature for ablation and does not cause serious injury to blood vessels and nerves. The NanoKnife electrode probe is 19G/1.2mm, and up to 6 ablation needles can be connected to the NanoKnife electrode probe, with the use of cardiac synchronizer. The ablation parameters usually used by NanoKnife are electric field strength 1500-3500V/cm, pulse width 70us-20ms, frequency 0.1-1Hz, pulse number 1-100. NanoKnife ablation also has certain defects like other tumor treatment technologies, such as: it is difficult to completely ablate lesions above 4cm, the procedure needs to be performed under general anesthesia, and it is impossible for patients with poor cardiopulmonary function, arrhythmia and cardiac pacemaker implantation. For patients with poor cardiopulmonary function, heart rate disorders and pacemaker implantation, ablation cannot be performed. After all, NanoKnife is a local tumor ablation technology, and it cannot replace systemic treatment for patients with extensive tumor metastasis. Nano knife ablation requires interventional doctors to have high technical skills in puncture and needle placement, because the intensity and uniformity of electric field are best when the nanoknife probes are parallel and spaced properly, in order to form a good DC high voltage electric field to penetrate the cell membrane and cause cell death, otherwise the ablation effect is greatly reduced. In order to achieve complete ablation of different types of tumors, there are also differences in the setting of treatment parameters. Due to the inhomogeneity of tissue structure and electrical conductivity, the so-called electric field depression can be formed in the ablation area, leading to incomplete inactivation of tumor cells during nanoknife ablation. Although one of the major advantages of nanoblade ablation is that it does not generate heat, thermal damage can still occur around the probe when the current is too high during ablation, and it is also important to be vigilant in clinical applications. As a new type of non-thermal tissue ablation technology, nanoknife will become an important supplement to conventional ablation in tumor treatment due to the advantage of selective protection of important human structures.