It is recommended that you take the test on an empty stomach in the morning to check the sex hormone six. Sex hormones include estradiol, progesterone, luteinizing hormone, follicle stimulating hormone, prolactin and testosterone. Among them, prolactin is easily affected by many factors, such as mood, exercise, hunger, eating, etc. It also has a rhythmic nature related to sleep, gradually rising after sleep, reaching the highest peak of the day in the morning before waking up, and then rapidly dropping to the lowest point after waking up, which is usually between 8 and 10 o’clock in the morning. Therefore, when checking sex hormone six, in order to avoid false values of prolactin, we suggest that it is best to check on an empty stomach in the morning. The significance of the sex hormone test is different in different time periods, so it is recommended to visit the hospital and standardize the test under the doctor’s guidance, so as to avoid false values due to time, thus affecting the standardized diagnosis and treatment.