Scalp hair follicle testing refers to the use of dermoscopy to examine the state of hair follicles by magnifying them. Scalp hair follicle detection is mainly carried out by dermoscopy. Dermoscopic diagnosis is a non-invasive microdiagnostic method developed in recent years, and in the diagnosis of hair disorders, it can provide information about the follicular units in the dermal openings, the microstructure of the dermal surface, the morphology of the hair shaft, the capillaries and the morphology of the hair root. Common hair loss disorders have characteristic dermoscopic signs associated with pathological changes, which can effectively help analyze the specific etiology and contribute to the treatment of scalp disorders in patients. Scalp follicle testing is a painless procedure that does not require anesthesia and does not cause damage to the patient’s skin, and there is no recovery period in the later stages, which needs to be performed under the operation of a physician.