Non-gonorrhea is non-gonococcal urethritis, and its examination includes mycoplasma and chlamydia detection, etc. Mycoplasma detection has culture method, PCR method, etc., and chlamydia detection has culture method, cytologic examination method, etc. 1. Mycoplasma detection (1) Culture method: the clinical culture method is generally not used to detect mycoplasma of the reproductive tract, and is only used for scientific research. At present, the clinical culture method can detect Mycoplasma urealyticum and Mycoplasma hominis. (2) Polymerase Chain Reaction (PCR) method: PCR reaction is extremely sensitive and is a more effective method of detection, but the experimental conditions, reagents and operators are more demanding, and improper operation is likely to cause misjudgment. (3) Serological tests: enzyme-linked immunosorbent assay and micro-immunofluorescence. However, since mycoplasma has no cell wall, the immunogenicity is weak and antibody formation is not significant enough, so the accuracy of the results is affected. 2. Chlamydia detection (1) Culture method: cell culture is the gold standard for Chlamydia trachomatis, but it is expensive, technically difficult and has a long cycle. (2) Cytological examination method: Chlamydia protozoa and inclusion bodies can be seen in the infected cells, cell specimens collected from the site of infection to make a smear, Giemsa stained inclusion bodies show blue or dark purple, iodine staining was brown. However, the sensitivity of this method is poor, has been less used. (3) Molecular biology technology testing: it is of great significance, including ligase chain reaction and transcription-mediated amplification test, enzyme-amplified immunoassay, polymerase chain reaction, nested polymerase chain reaction and so on. Patients with non-gonorrhea should go to the hospital in time to improve the relevant examination and standardize the treatment under the guidance of the doctor.