Glutathione, currently, has two isozymes, one located in the cell plasma and the other in the mitochondria. The intracytoplasmic glutathione isozyme, also known as supernatant glutathione, is more likely to be released into the blood after cellular injury. Therefore, this isoenzyme appears to be elevated earlier and may be elevated more rapidly. The glutathione isoenzyme located in the mitochondria, also known as mitochondrial glutathione, is more tightly bound and will only gradually increase when there is severe cell damage or cell necrosis. Therefore, it is also necessary to determine the cause of the high glutathione isoenzyme according to the specific type of glutathione isoenzyme in clinical practice. If only the supernatant glutathione is high, it may be caused by either physiological factors or disease. If mitochondrial glutathione is high, it is likely to be caused by diseases such as myocarditis, myocardial infarction, liver cirrhosis and hepatitis.