A positive TRUST test does not confirm a diagnosis of syphilis. A positive TRUST test result for syphilis usually indicates that non-specific antibodies to syphilis, also known as a positive Toluidine Red Unwarmed Serologic Test (TRUST), were detected in the test specimen, suggesting that a syphilis infection may be present. However, if the diagnosis is to be confirmed, further testing for syphilis-specific antibodies is needed as a confirmatory test. After the syphilis spirochete infects the human body, the body’s immune system will produce a lot of antibodies against the spirochete, and there are two types of antibodies: one is specific antibodies called TP antibodies, and the other is non-specific antibodies called anticardiolipin antibodies or plasma reactive elements. TRUST positivity can be expressed in terms of titer, and the titer can reflect the activity of syphilis disease and the contagiousness of syphilis. Therefore, if the test is positive for TRUST, it should be followed up with a test for syphilis-specific antibodies. If the specific TP antibody is positive, then the diagnosis of syphilis can be confirmed. Otherwise, if the test is simply positive for TRUST but negative for TP antibody, the test will be a false positive, and the diagnosis of syphilis cannot be made.