HPV16 positive has been 20 years first through the cervical tct examination to rule out cervical intraepithelial neoplasia and cervical cancer, ruled out for HPV16 positive usually take the general treatment, drug treatment, physical therapy and so on. 1. General treatment: HPV16 has been positive for 20 years, which means that the autoimmunity is poor, and it is impossible to make HPV turn negative on its own. It is recommended that patients develop a good lifestyle, appropriate exercise, regular work and rest, and eat more food rich in high quality protein and dietary fiber to enhance the body’s resistance. 2. Medication: Topical imiquimod, fluorouracil and other drugs can be used to carry out local antiviral treatment; interferon, thymidine and other drugs can also be used to improve the body’s immune ability to fight diseases. 3. Physiotherapy: If the patient’s cervix has already developed lesions, laser, freezing, surgery and other ways of treatment can be carried out to avoid adverse consequences. If the patient has this phenomenon, it is recommended to maintain a positive attitude, pay attention to daily hygiene and cleanliness, so as to avoid aggravation or deterioration of the condition. If necessary, timely medical treatment, follow the doctor’s instructions, scientific use of drugs.