Nucleotidases in the serum are widely found in the liver tissue. If a patient has high serum nucleotidase, it is likely that the patient has hepatobiliary system diseases, especially for hepatobiliary system biliary tract obstruction diseases and tumor diseases, which have specific diagnostic significance. In addition, nucleotidase can be combined with alkaline phosphatase to determine the patient’s hepatobiliary system disease. If the patient has high alkaline phosphatase and nucleotidase at the same time, the patient should be considered to have obstructive biliary tract disease, and further ultrasound of the upper abdomen or MRI of the upper abdomen is needed to determine the cause of high nucleotidase. If the patient only has elevated alkaline phosphatase and normal nucleotide enzymes, the patient should be considered to have diseases of extrahepatic organs, such as rickets or chondromalacia, which can cause simple elevation of alkaline phosphatase. In addition, if the nucleotide enzyme is elevated, the patient may have viral hepatitis, cirrhosis, primary liver tumors, or inflammation of the pancreas or bile ducts.