Hepatitis B Virus Surface Antigen Test includes configuring liquid, numbering, adding sample, adding enzyme, setting temperature, washing, and color development. 1. Liquid configuration: Dilute the concentrated washing solution with distilled water and prepare for use. 2. Numbering: Arrange the samples corresponding to the microtiter wells in serial numbers and set up the control group according to the grouping of negative, positive and blank. 3. Add sample: add the sample to be detected in the microwells. 4. Add enzyme: add enzyme reagent into the microwells. 5. Setting temperature: set the temperature to 37℃ for 30 minutes after sealing the plate. 6. Wash: Use an attractor to suck up the liquid in the microtiter wells. 7. Color development: add the color developer into the microtiter wells and mix well, then measure the OD value of the microtiter wells by enzyme marker. Sample OD value ≥ critical value is positive. Sample OD value ≤ critical value is negative. If you want to know more, it is recommended to consult a professional doctor.