Hepatitis B carriers with negative DNA quantification have the potential to become positive. Hepatitis B carriers are those who carry the hepatitis B virus without any impairment of liver function. Hepatitis B DNA can be negative or positive in this group of patients. The amount of virus in the body changes accordingly to the virulence of the virus and the resistance of the body. When the resistance decreases and the virulence of the virus is stronger, the DNA quantification may change from negative to positive. The lower the DNA quantification of hepatitis B carriers, the less infectious they are, and the higher the DNA quantification of hepatitis B, the more active the virus replicates and the more infectious it is. Hepatitis B carriers also need to go to the hospital for regular checkups. If the DNA quantification changes from negative to positive, it is necessary to pay attention to whether there is active viral replication affecting the liver function, so as to treat the symptoms.