1.Urinary routine: Retrieve clean mid-stage urine. Female patients should avoid menstruation, wash the vulva and keep the urine line constantly, and directly receive about 10ml of middle segment urine. 2.24-hour urine protein quantification, 24-hour urine creatinine, 24-hour urine electrolytes: urinate at 7:00 am and discard, after that all urine is kept in a container and placed in a cool place (if necessary, add the corresponding drugs according to the doctor’s prescription) until 7:00 am on the second day, and the last urine is kept in a container. After the container, the volume of urine was measured accurately with a measuring cup for 24 hours, and 10ml was kept after mixing and sent for testing. 3, urine erythrocyte phase difference microscopy: about 10ml of fresh urine is retained, and the specimen is sent for examination immediately after retention. Female patients should avoid menstrual period. 4, urine bacterial culture: wash the vulva (if necessary, disinfection), open the urine culture bottle, hands do not touch the inside of the bottle mouth and the inside of the bottle stopper, keep the urine line, about 10ml of specimens can be directly retained, cork the bottle tightly for testing.