Some kidney diseases often require a “24-hour urine protein quantification” test, so how to collect and send the urine specimen? In order to accurately measure the 24-hour urine protein quantification, the urine in the bladder should be emptied and discarded at 8:00 a.m., start timing, and store all the urine excreted for 24 hours in a container (including the urine quasi-dissolved at 8:00 a.m. the next day) and send it all for examination. If you have a bowel movement within 24 hours, you should first collect the urine and then remove it. Small volumes of urine should also not be missed. If the urine volume is not collected completely, the calculation of urine protein volume will not be accurate. Before the test, we should measure the total amount of urine in a measuring cup, then stir it well, take out a small cup to determine the amount of protein per 100 ml, and then calculate it according to the actual amount of urine, and we can calculate the amount of protein for 24 hours. In conclusion, to accurately measure the 24-hour urine protein amount, the entire day’s urine volume must be accurately collected and the test portion must be a mixture of the total urine volume in order to be representative. Because this test is to calculate the absolute value of urine protein, it has little to do with the amount of water consumed, so it is not necessary to restrict water and food intake on the day of measurement, and eating as usual is sufficient.