Currently there is no better self-test for the diagnosis of Toxoplasma gondii. At the present stage, the main methods for diagnosing Toxoplasma gondii infection are pathogenetic and serologic tests, as well as molecular diagnostic techniques. Among them, the pathogenic examination has a confirmatory significance, and can mainly rely on the smear staining method, which can take ascites, pleural effusion, amniotic fluid, cerebrospinal fluid, bone marrow or blood of patients in the acute stage, and then centrifuged to take the sediment to make a smear, or use the smear of biopsy material to microscopically detect toxoplasma gondii trophozoites. However, this method is simple, but the positive rate is not high and easy to miss detection. Because of the difficulty and low positive rate of toxoplasma pathogenetic examination, serologic examination is an important auxiliary diagnostic tool that is widely used nowadays. At present, commonly used staining test for determination, such as indirect hemagglutination test, the method has good specificity and sensitivity, simple operation, suitable for epidemiological investigation, widely used. In addition to this, molecular diagnostic techniques with high sensitivity and specificity can also be used, such as the use of real-time fluorescence quantitative amplification technology for the detection of Toxoplasma gondii, commonly used target B1 gene, for the detection of amniotic fluid specimens suspected of congenital infection.