The main difference between taking a sample through the nose and the oropharynx for nucleic acid testing is the route, location, accuracy of the test, and discomfort caused to the body. Nucleic acid testing is done by taking a specimen through our nose or pharynx (voice) using a pharyngeal swab for nucleic acid testing of novel coronaviruses. Neocoronaviruses are respiratory viruses, so the chances of the virus being present in the respiratory tract are the highest. Entry through the nose may be painful and even bleed easily, but the site of arrival may be a little deeper, and some people will experience significant foreign body irritation, and may even have an uncomfortable reaction such as itchy nose with sneezing. Sampling from the pharynx (throat) is generally easy for patients to accept, but it is often difficult to reach the posterior pharyngeal wall due to the nausea reflex that occurs when the palatal pituitary and the root of the tongue are stimulated, and the sample is easily interfered with by a number of other factors such as saliva. Choosing upper respiratory tract testing, either from the nasal cavity or the pharynx, has the advantages of simplicity, convenience and speed. As to how far the accuracy of the two sampling methods differs, current research is inconclusive, but both are recognized and recommended by the clinic, so please refer to the recommendations of the sampling site for specific methods.