It has been reported that 12%-25% of DM patients have foot ulcers, and diabetic foot can develop rapidly, and if not handled properly (especially when combined with infection) can lead to aggravation of the disease, and in severe cases, disability or even death. The independent factors of diabetic foot, causing infections such as sensory and autonomic neuropathy, microvascular and macrovascular lesions and immune factors, play an important role in local tissue necrosis, especially when trauma is more likely to occur, all layers of skin, tissue, joints and bones can be involved, leading to gangrene and eventual amputation in severe cases. In the United States and the United Kingdom, the diabetic foot is the leading cause of hospitalization in patients with DM. Qian Rongli et al. mouth1 reported that the prevalence of lower limb vascular complications in patients hospitalized in endocrinology reached 9.3%. O3, has a powerful bactericidal effect, as early as 1915 Wolff et al. used O3, local treatment of serious injury 121 infection, the mechanism is O3, easy to combine with unsaturated fatty acids in bacterial cell membranes, oxidize intracellular enzyme proteins or RNA, DNA, change cell permeability, inactivate them, leading to bacterial lysis and death. o3, is a strong oxidant, its ability to activate red blood cells, promote red blood cell O3, a strong oxidant, activates red blood cells, promotes the release of oxygen, increases the amount of oxygen carried by blood and tissue oxygen; enhances the deformability of red blood cells and improves blood rheology; O3, easily soluble in water, increases the content of dissolved oxygen in blood and raises the partial pressure of oxygen, which is conducive to improving tissue hypoxia in the body. O3, which enters the cavity or touches the body surface, can supply oxygen to the ulcerated tissue while anti-inflammatory and anti-infective, promoting wound healing. Larini et al. found that O3, activates immunologically active cells, increasing the release of cytokines such as interferon, interleukin, tumor necrosis factor, granulocyte macrophage colony-stimulating factor, and enhancing the body’s immune function. O3, concentration is the key to determine the success of cytokine induction, 20-40ug/ml of O3. concentration is the effective concentration range for immune activation. the mechanism of O3, action on immune cells is not yet clear, it may be that the therapeutic concentration of reactive oxygen clusters drives the blood to produce lipid peroxides and O3, like peroxides, these reactive peroxides enter the cytoplasm, activating nuclear factor NF.kB, which causes transcription and translation of cellular genes and release of cytokines, acting as anti-inflammatory and enhancing the immunity of the organism. It is also reported that O3, can induce and activate the body’s antioxidant enzyme system, producing a large number of superoxide dismutase, catalase, glutathione peroxidase and reductase, scavenging the body’s excessive free radicals. The animal model of DM showed that O3 can reduce oxidative stress, improve vascular endothelial function, and stall the progression of large and small vessels and neuropathy in DM. In addition, O3 can lower cholesterol, inactivate inflammatory factors, and have analgesic effects. tafil-Klawe et al. reported that the treatment of chronic skin ulcers with O3, air bath therapy is simple, convenient, safe, and effective, and can significantly reduce the rate of amputation. The results of this study showed that the wound healing rate in the treatment group was significantly higher than that in the control group, and the amputation rate was significantly lower than that in the control group. This indicates that O3, the treatment of refractory diabetic infected foot ulcers in a confined environment has good anti-infection, reduces trauma exudation and promotes trauma healing.