Not sleeping well at night generally does not affect the results of the Mid-Tang, if the results of the Mid-Tang is abnormal, but also need to follow the doctor’s advice to improve further examination. Down syndrome screening refers to the screening of pregnant women for Down syndrome in the mid-pregnancy (15-20 weeks), which can help pregnant women assess whether the fetus is suffering from chromosomal abnormalities such as Down syndrome and the risk of open neural tube defects, so as to improve the rate of eugenic sterilization. Down syndrome screening requires a blood test, and under normal circumstances, a pregnant woman’s lack of sleep at night will not affect the results of the CTS. If the test results are abnormal, it usually suggests that the fetus may have chromosomal abnormalities and genetic syndromes such as Down syndrome. However, because the test results are affected by the pregnancy week and the age of the pregnant woman, pregnant women should go to the hospital in time and follow the doctor’s instructions to improve the next step of the examination to identify and diagnose.