Orofacial pain is one of the most common clinical conditions, and patients often complain of localized skin, bone, or dental pain. The main types are “odontogenic pain, temporomandibular joint disorders, trigeminal neuralgia, glossopharyngeal neuralgia, occlusal phantom syndrome”. Ozone (O3) is an allotropic isomer of oxygen, a molecule consisting of three oxygen atoms with a ring-like structure. Medical ozone generator through the use of medical oxygen through the high voltage to generate ozone, ozone is a light blue gas with a strong special odor, extremely unstable, in the air and human tissue is easily decomposed into ○2 + ○ -. Compared with oxygen, ozone has the characteristics of large specific gravity, smell, color, soluble in water, easy to decompose, etc. The half-life is about 20 minutes at room temperature. Since ○-atoms are very active, ozone has strong oxidizing ability and biological effects such as bactericidal, antiviral, anti-inflammatory, analgesic and immunomodulation, and is widely used in clinical practice. In the field of pain, whether it is odontogenic pain, neurogenic pain, or temporomandibular joint inflammatory pain, local injection of ozone for the treatment of orofacial pain is a new therapeutic method that has emerged in recent years, which utilizes the strong oxidative property of ozone. The specific mechanisms are as follows: ① ozone can stimulate the overexpression of oxidative enzymes, neutralize reactive oxidation products, antagonize cytokines, cause vasodilatation, and promote the absorption of inflammation; ② ozone acts on the peripheral nerve endings of muscles or joints to stimulate the release of enkephalins from inhibitory interneurons, etc., to achieve the effect of analgesia; ③ ozone can change the environment of the muscles and the joint cavities to promote the repair and regeneration of the cartilage and to prevent or delay the degenerative changes of the joints. (iii) Ozone can change the environment of muscle and joint cavity, promote cartilage repair and regeneration, and prevent or delay joint degenerative changes. Therefore, ozone can be injected into local tissues or joint cavities to eliminate local inflammation, promote tissue repair, inhibit the release of related pain factors and reduce pain. In recent years, ozone therapy has become a more and more widely used therapeutic method in clinical diagnosis and treatment. This non-mainstream medical treatment method can replace the main methods that are limited by the dosage of medicinal preparations, as well as those that are not suitable for the conventional treatment of various pathological conditions, and can help to stabilize the self-regulatory balance that has been damaged, improve the functional status of various organs and systems, and activate the body’s self-protection ability. The use of ozone therapy is very common abroad. In a series of international professional conferences in recent years, a large amount of experimental and clinical data on the use of triple-oxygen therapy has been published and accumulated, clearly indicating the medical utility of triple-oxygen therapy from both positive and negative sides, and determining the basic method of using triple-oxygen therapy, so that the parameters of the original therapeutic method are more clearly defined.