Persistent positivity for high-risk human papillomavirus 16 in women is a high-risk factor for cervical cancer. It takes about a few years or even a decade from the time the virus is contracted until it develops into cervical cancer. Therefore, it is the persistent infection of high-risk HPV16 positive that may induce cervical cancer. Secondly, after HPV16 infects the cervical squamocolumnar junction zone, it does not cause cervical cancer in the first place, but cervical intraepithelial neoplasia, which causes cytological changes and is primary at first, such as grade 1, and then slowly escalates to grade 2 or 3 without treatment before it transforms into cervical invasive cancer.